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| For
current research activities, please click here: Research
Programs |
| 1978-81 |
Conducting research for Ph.D. thesis in the Department
of Experimental Pathology, University of California at San Francisco,
under the instruction of Dr. Robert Stern and Dr. Denis Gospodarowicz,
on the study of collagen biochemistry and endothelial cell biology
affected by fibroblast growth factor and extracellular matrix. |
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| 1981-82 |
Conducting research at the Wilmer Institute, and
the Department of Dermatology, the Johns Hopkins Hospital, under
the supervision of Dr. Tung-Tien Sun, on the study of the functional
role of different keratin antigens in epithelial differentiation,
in embryonic development of skin and cornea, and in abnormal
keratinization secondary to vitamin A deficiency using a monoclonal
antibody approach. |
|
| 1982-84 |
Conducting research in the laboratory of Eye Pathology,
the Wilmer Institute, under the supervision of Dr. W. Richard
Green and Dr. A. Edward Maumenee, on the study of conjunctival
goblet cell biology, including the role of retinoids in modulating
conjunctival transdifferentiation, development of a modified
impression cytology technique for clinical studies of squamous
metaplasia, and the study of clinical efficacy of topical retinoids
in treating various ocular surface disorders. |
|
| 1984-86 |
Conducting research in the laboratory of Dr. Ilene K. Gipson
on the action mechanism of various retinoids in the modulation
of conjunctival goblet cell differentiation, and on the development
of monoclonal antibodies against conjunctival mucins.
Under the supervision of Dr. Kenneth R. Kenyon, conducting
research on conjunctival goblet-cell density and distribution
in rats in relation to the aging process and vitamin A deficiency.
Applying the impression cytology technique in clinical and
basic research of various ocular surface disorders, including
dry-eye syndrome, blepharitis, vitamin A deficiency, alkaline
burns, and etc.
Evaluating the clinical efficacy of topical retinoids in
the treatment of various ocular surface disorders. Organizing
the multi-center clinical trial of topical tretinoin for severe
dry eye disorders.
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|
| 1986-01 |
Supervising researches in the areas of cell biology,
tissue culture, biochemistry and molecular biology. |
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| Specific research
areas include |
| • |
Establishment of various epithelial cell
cultures, |
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| • |
Factors modulating goblet cell differentiation, |
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| • |
Molecular mechanism for the tear film stability
in the interface between mucin and ocular surface membrane
proteins, |
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| • |
Modulation of epithelial differentiation
by retinoids, |
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| • |
Development of autologous and allogeneic
limbal epithelium stem cell transplantation for limbal
stem cell deficiency including chemical burn, aniridia,
and Stevens Johnson syndrome |
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| • |
Exploration of stem-cell regulation for
corneal and conjunctival epithelia, |
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| • |
Studies of mechanism of photothrombosis
mediated by rose Bengal. |
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| • |
Explore the mechanism why rose bengal does
not stain normal ocular surface epithelia but stains abnormal
squamous metaplastic epithelia, |
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| • |
Development of monoclonal antibodies to
cornea-specific keratin K12 and mucosal epithelial membrane-associated
mucin, |
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| • |
Studies of cytokine network between ocular
surface epithelium and fibroblasts, |
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| • |
Cytological characterization of limbal stem
cell deficiency, |
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| • |
Studies of the pathogenesis of corneal diseases
characterized by limbal stem cell deficiency including
chemical injuries, |
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| • |
Development of new surgical procedures of
amniotic membrane transplantation for ocular surface reconstruction,
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| • |
Studies of the regulatory mechanism of limbal
stem cell functions, |
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| • |
Exploration of cytokine network responsible
for corneal scarring. |
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| • |
Initiating a tissue matrix therapy program
using amniotic membrane transplantation for ocular surface
reconstruction, |
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| • |
Exploring the mechanism by which fibrovascular
growth with corneal invasion occurs in pterygium. |
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| • |
Exploring and perfecting the new strategy
of ocular surface reconstruction using ex vivo expansion
of epithelial stem cells on amniotic membrane cultures.
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|
| 2002 |
| • |
Developing a research program of tissue
engineering to promote epithelial tissue healing and ocular
surface reconstruction. |
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| • |
Characterizing dry eye according to the
lipid tear status using kinetic tear interference images, |
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| • |
Development of new lipid replacement therapy
for lipid tear deficiency, |
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| • |
Developing and tissue engineering of sutureless
AmnioLens, |
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| • |
Starting FDA phase I clinical trial: Transplantation
of Ex Vivo Expanded Limbal Epithelial Stem Cells"
for patients suffering total limbal stem cell deficiency. |
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